Concurrent Prebiotic Intake Reverses Insulin Resistance Induced by Early-Life Pulsed Antibiotic in Rats

Klancic, Teja and Laforest-Lapointe, Isabelle and Wong, Jolene and Choo, Ashley and Nettleton, Jodi E. and Chleilat, Faye and Arrieta, Marie-Claire and Reimer, Raylene A. (2021) Concurrent Prebiotic Intake Reverses Insulin Resistance Induced by Early-Life Pulsed Antibiotic in Rats. Biomedicines, 9 (1). p. 66. ISSN 2227-9059

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Abstract

Pulsed antibiotic treatment (PAT) early in life increases risk of obesity. Prebiotics can reduce fat mass and improve metabolic health. We examined if co-administering prebiotic with PAT reduces obesity risk in rat pups weaned onto a high fat/sucrose diet. Pups were randomized to (1) control [CTR], (2) antibiotic [ABT] (azithromycin), (3) prebiotic [PRE] (10% oligofructose (OFS)), (4) antibiotic + prebiotic [ABT + PRE]. Pulses of antibiotics/prebiotics were administered at d19–21, d28–30 and d37–39. Male and female rats given antibiotics (ABT) had higher body weight than all other groups at 10 wk of age. The PAT phenotype was stronger in ABT males than females, where increased fat mass, hyperinsulinemia and insulin resistance were present and all reversible with prebiotics. Reduced hypothalamic and hepatic expression of insulin receptor substrates and ileal tight junction proteins was seen in males only, explaining their greater insulin resistance. In females, insulin resistance was improved with prebiotics and normalized to lean control. ABT reduced Lactobacillaceae and increased Bacteroidaceae in both sexes. Using a therapeutic dose of an antibiotic commonly used for acute infection in children, PAT increased body weight and impaired insulin production and insulin sensitivity. The effects were reversed with prebiotic co-administration in a sex-specific manner.

Item Type: Article
Uncontrolled Keywords: antibiotic; prebiotic; oligofructose; insulin resistance; obesity; gut microbiota
Subjects: STM Repository > Biological Science
Depositing User: Managing Editor
Date Deposited: 17 Oct 2024 04:32
Last Modified: 17 Oct 2024 04:32
URI: http://classical.goforpromo.com/id/eprint/1022

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