A Portable, Label-Free, Reproducible Quartz Crystal Microbalance Immunochip for the Detection of Zearalenone in Food Samples

Liu, Shengmiao and Liu, Xinyu and Pan, Qianwen and Dai, Zhihan and Pan, Mingfei and Wang, Shuo (2021) A Portable, Label-Free, Reproducible Quartz Crystal Microbalance Immunochip for the Detection of Zearalenone in Food Samples. Biosensors, 11 (2). p. 53. ISSN 2079-6374

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Abstract

This research reports a portable immunochip, based on quartz crystal microbalance (QCM) for label-free, low-cost qualitative detection of zearalenone (ZEN) in food samples. The experimental parameters in the functionalization and working process were evaluated in detail, in order to achieve a high accuracy and sensitivity. Under optimal conditions, the ZEN concentration at an inhibition ratio of 50% and 15% of the proposed QCM immunochip achieved 3.41 µg L−1 and 0.37 µg L−1, respectively. This portable QCM immunochip also exhibited high specificity, no obvious cross-reaction to five structural analogs of ZEN, and showed other mycotoxins. It could finish the whole qualitative measurement within 30 min, showed good stability during the processes of preparation (SD < 5%, n = 9), storage (frequency response >90%, in PBS at 4 °C for 15 days), and application (frequency response >90% after being reused 6 times). The developed QCM immunochip obtained accurate and repeatable recovery results in ZEN analysis in the chosen food samples (corn, wheat flour, soy sauce, and milk), which had a high correlation (R2 = 0.9844) with that achieved by the HPLC–MS/MS method. In short, this work developed a portable, stable, and reproducible QCM immunochip that could be used for rapid, low-cost, and sensitively measurement of ZEN content in real food samples.

Item Type: Article
Uncontrolled Keywords: zearalenone; quartz crystal microbalance; portable and reproducible immunochip; label free detection
Subjects: STM Repository > Agricultural and Food Science
Depositing User: Managing Editor
Date Deposited: 02 Dec 2024 09:54
Last Modified: 02 Dec 2024 09:54
URI: http://classical.goforpromo.com/id/eprint/1222

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