Liu, Shengmiao and Liu, Xinyu and Pan, Qianwen and Dai, Zhihan and Pan, Mingfei and Wang, Shuo (2021) A Portable, Label-Free, Reproducible Quartz Crystal Microbalance Immunochip for the Detection of Zearalenone in Food Samples. Biosensors, 11 (2). p. 53. ISSN 2079-6374
biosensors-11-00053-v2.pdf - Published Version
Download (2MB)
Abstract
This research reports a portable immunochip, based on quartz crystal microbalance (QCM) for label-free, low-cost qualitative detection of zearalenone (ZEN) in food samples. The experimental parameters in the functionalization and working process were evaluated in detail, in order to achieve a high accuracy and sensitivity. Under optimal conditions, the ZEN concentration at an inhibition ratio of 50% and 15% of the proposed QCM immunochip achieved 3.41 µg L−1 and 0.37 µg L−1, respectively. This portable QCM immunochip also exhibited high specificity, no obvious cross-reaction to five structural analogs of ZEN, and showed other mycotoxins. It could finish the whole qualitative measurement within 30 min, showed good stability during the processes of preparation (SD < 5%, n = 9), storage (frequency response >90%, in PBS at 4 °C for 15 days), and application (frequency response >90% after being reused 6 times). The developed QCM immunochip obtained accurate and repeatable recovery results in ZEN analysis in the chosen food samples (corn, wheat flour, soy sauce, and milk), which had a high correlation (R2 = 0.9844) with that achieved by the HPLC–MS/MS method. In short, this work developed a portable, stable, and reproducible QCM immunochip that could be used for rapid, low-cost, and sensitively measurement of ZEN content in real food samples.
Item Type: | Article |
---|---|
Uncontrolled Keywords: | zearalenone; quartz crystal microbalance; portable and reproducible immunochip; label free detection |
Subjects: | STM Repository > Agricultural and Food Science |
Depositing User: | Managing Editor |
Date Deposited: | 02 Dec 2024 09:54 |
Last Modified: | 02 Dec 2024 09:54 |
URI: | http://classical.goforpromo.com/id/eprint/1222 |