Assessment of aluminum tolerant of double haploid lines for developing new type of upland rice

Aluminum can possibly have direct or indirect adverse effects on plant growth; however, this effect is not the same for all plants, even in the same species. The roots of plants are most sensitive to Al toxicity accompanied to initial symptoms such as the inhibition of cell extension and retarded development of root systems. This study was aimed to evaluate doubled-haploid (DH1) upland rice derived from anther culture to Al stress and to study the genetic diversity and population distribution of DH lines due to Al stress. Al tolerant test was carried out in a greenhouse using factorial Randomized Complete Block Design (RCBD) with three replicates. Yoshida nutrient solution containing Al of 0 and 45 ppm was the first factor, while the second was the lines obtained from previous experiments (DH1), the four parents (SGJT36, SGJT28, Fatmawati, and Way Rarem), while Dupa, and ITA131, respectively as an Al tolerant and susceptible checks.. The results showed that root length, shoot length, and shoot dry weight had high heritability values and correlated well with the observed characters. Al tolerant doubled haploid upland rice lines derived from anther culture varied widely. Based on the Relative Root Length (RRL), out of 58 lines tested, 19 genotypes were highly tolerant, 29 lines were moderate, while 10 were low. The DH1 rice derived from P3 showed high, moderate, and low tolerance, while those from P6 showed high and moderate tolerance only.