In vitro Rapid Regeneration of Betel Vine (Piper betle L.)

The experiment was conducted on betel vine (Piper betle L.) for in vitro regeneration in Biotechnology Lab. of the Department of Biotechnology, Sher-e-Bangla Agricultural University, Bangladesh from the period of July 2014 to April 2015 to investigate the effect of BA, KIN, IAA and IBA in MS medium, where nodal segment was used as explants. The highest percent of shoot proliferation (70%) was achieved with 1.5 mg/L BA + 3.0 mg/L KIN whereas the highest number of shoots and leaves were obtained in 1.0 mg/L BA + 3.0 mg/L KIN. The maximum 14.40 roots/explants were observed with 3.0 mg/L IBA compare with IAA. For hardening the regenerated plantlets were transferred to growth chamber where maximum survival rate (90%) was observed whereas 75% plantlets were survived in shade house in soil which was higher than in open atmosphere (50%).This experiment can be used as a reliable protocol for in vitro regeneration of betel vine.