Second-Order Derivative Spectrophotometric Estimation of Magnesium and Titanium Using 3-Aminopyridine-2-Carbaxal Dehydethiosemicarbazone

The aim of the present study deals with the experimental and theoretical aspects of Derivative UV-Spectrophotometry. The method gains significance using the first and second derivative of the transmission spectra with respect to wavelength. Generated optical derivatives are compared to the known numerical derivatives. Using the second-order derivative spectrophotometric method, the determination of magnesium [Mg (II)] and titanium [Ti (IV)] using 3-aminopyridine-2-carboxaldehyde thiosemicarbazone (triapine) has been examined. Triapine, when coupled with Mg(II) and Ti(IV), yields a light green hue in a basic solution (at pH 8). The prominent peak was between 370 and 380 nm for Mg(II) and Ti(IV) compounds. Molar absorption and Sandell's sensitivity values of the coloured complexes viz., Mg(II) and Ti(IV)-triapine complexes were 9.269 x 106 L mol-1 cm-1, 7.96 x 103 L mol -1 cm -1, and 0.0001 g/cm2, 0.125 g/cm2, respectively. Linearity is observed in the concentration range 0.121-1.318 g/mL for Mg (II) and 0.012-0.122 g/mL for Ti(IV). The concentration effect of Mg(II) and Ti(IV) ions on amplitude are also studied. The limit of detection (LOD) and the limit of quantification (LOQ) of Mg(II) and Ti(IV) are 0.00495 g/mL, 0.015 g/mL and 0.007 g/mL, 0.0022 g/mL respectively. The stability constant of the Mg(II) and Ti(IV)-triapine complex is determined by the Jobs method to be 4.260 x 104 and 3.236 x 105, respectively. Derivative spectrophotometry as a technique which allows to non-invasive extraction of data included in basic spectrum appears to be a very valuable tool in physico-chemical studies. It permits to investigate the reaction equilibria or kinetics without disturbing their run. Here in this chapter, a multicomponent analysis of Mg-Ti alloy samples using tripin as a chelating agent and derived second-order spectrophotometry was developed. Magnesium-Titanium Alloys have been identified in normal alloy steel, mineral, pharmaceutical, and food samples using the suggested method. The findings of the current study samples are in good agreement with those of the food analysis in atomic absorption spectroscopy (F-AAS) and inductively coupled plasma atomic emission spectroscopy (ICP-AES).