Phytochemical Analysis by HR-LCMS and In-vitro Anti - diabetic Potential of Michelia champaca Bark

The current study was aimed to explore the primary phytochemicals and HRLCMS was performed to separate and isolate the phytoconstituents present in Michelia champaca established on their retention time. Natural products are organic molecules with a variety of structural properties and biological activities that have evolved via long-term natural selection and evolution. They are safer and more affordable than synthetic products. The plant specimen for the proposed report was accumulated from Tirupati District of Andhra Pradesh in the month of February 2018. The outcomes of HR-LCMS showed the presence of 15 phytochemical compounds. DL-Carnitine, Catechin, D- -Tocopherol, Colchicine, Myricetin, Epicatechin, Quercetin, Epigallocatechin gallate, Quercetin-3 -D-glucoside, Kaempferol, Sorbic acid, Apocynin, Epigallocatechin gallate, myricetin 3-O-beta-D-galactopyranoside, Naringeninchalcone are the main compounds identified. In the experimental models, the inhibition of enzymes such as -amylase and -glucosidase delays the rate of glucose absorption and lowers blood glucose levels. In HR-LCMS elucidation, the compounds like DL-Carnitine, Catechin, D- -Tocopherol, Colchicine, Myricetin, Epicatechin, Quercetin, Epigallocatechin gallate, Quercetin-3 -D-glucoside, Kaempferol, Sorbic acid, Apocynin, Ellagic acid, myricetin 3-O-beta-D-galactopyranoside, Naringeninchalcone were present in Michelia champaca (Bark). The IC50 values of -amylase and -glucosidase inhibitory activity of HEMC was acquired to be 88.65 g/mL and 71.28 g/mL correspondingly. Positive control acarbose displayed IC50 value of 52.94 g/mL and 50.01 g/mL correspondingly. The present data insinuated that the extracts possess direct and potent antioxidant activities through multiple mechanisms.