BIOLOGICAL AND GENETIC OVERVIEW OF Pectinophora gossypiella AND ITS ASSOCIATED PARASITOID Bracon brevicornis GENOMES USING TWO MOLECULAR MARKERS TECHNIQUES

Bachground: The comparative between the long-term autumn storage Pectinophora gossypiella diapauses larvae (field strain) (durations extended to 4 months) with active larvae (laboratory strain) and its associated field parasitoid Bracon brevicornis were investigated.

Results: The mean duration of the total immature stages of B. brevicornis was 12.5 and 10.0 days, respectively, when reared on P. gossypiella diapaused larvae (field strain) and active laboratory strain. The generation time of B. brevicornis was 19.2 when reared on field diapaused larvae and 14.9 days for laboratory strain. The Sex ration of B. brevicornis estimated by (0.6:0.4) in active larvae and (0.55:0.45) in diapause larvae. The percentage of produced females in progeny was significantly different from that of two cultured parasitoids. Also, results revealed that adult male numbers decreased than female which considerable necessary in the B. brevicornis progeny production of following generations. The total eggs number per B. brevicornis female was 158.0 when reared on diapaused larvae of P. gossypiella field strain, while it increased to 236.0 when reared on active larvae of P. gossypiella laboratory strain. Identification of P. gossypiella and B. brevicornis genomes using two different techniques; SCoT method which conjugate with genes for the first time in Egypt on the host and its parasitoid and ISSR method which conjugate with sequences.

Conclusion: using the two techniques had been recognized the most parts of P. gossypiella and B. brevicornis genomes, our findings may be helpful in genetic and genomic studies.