Formulation Development of Naturally Sweet Herbal Oral Health Drinks and Its HPTLC Analysis

Ashwagandha, Tulsi, Mulethi, Awala, Shatavari, Gokharu, Arjun, Giloy, Safed musli, Kalimirchi, Haldi,
Jaiphal can be used as an active ingredients and aqueous extract of Stevia rebaudiana as natural
sweetener with nutraceutical in health dinks. The product can be developed by treating concentrates
of each crude drug with purified water. TLC profile, HPTLC method development and validation can
be carried out using Gallic acid as a standard. A new simple, sensitive, selective, precise and robust
HPTLC method for analysis of Gallic acid in health drink can be developed. Precoated silica health
drink aluminium plate 60F-254 (20 × 10 cm) with 200 μm thickness can be used as stationary phase
while toluene: ethyl acetate: formic acid: ethanol (6: 4: 0.3: 0.4) system can be developed as a mobile
phase. Spectrum analysis showed the same Rf values and spectrum pattern of standard and sample.
The method can be validated by using accuracy, precision, linearity, robustness, ruggedness and
recovery as applicable parameters. The developed method can be quite good and most sensitive for
the present products. The unpleasant and bitter taste of the product can be masked by different
concentrations of aqueous extract of Stevia. Sweetness potency can be determined by taste
evaluation method. 1% Stevia extract may be sufficient to produce most palatable and acceptable
sweet preparation.