In vitro INHIBITION OF PATHOGENIC BACTERIA Helicobacter pylori BY POTENTIAL PROBIOTIC BACTERIA Lactobacillus casei STRAINS

Aim of these objectives the antimicrobial effects of Lactobacillus casei strains were tested against 3 strains of H. pylori were used well diffusion assays method and co-culturing of Lactobacillus strain and H. pylori. Antagonistic effect of six different Lactobacillus spp. was determined against one type strain of H. pylori 11638 and two H. pylori 21175, H. pylori 3119299, respectively. Six good Lactobacillus strains L. casei DCM 1008, L. casei 16s MG3, L. casei BN11, L. casei MG3, L. casei H248 and L. casei LBR1 were selected for additional assessment. The unadjusted and neutralized supernatants collected from all six Lactobacillus spp showed inhibition against all three H pylori isolates. L. casei DCM 1008, MG3, BN11, MG3, LBR1 and H248 had shown better inhibition than the rest of Lactobacillus casei spp. Co-culturing of Lactobacillus casei, 16S isolate MG3, BN11 and H248 including all three H. pylori showed high inhibition against this pathogen. All strains of Lactobacillus produced adjustable concentrations of acetic acid and lactic acid.