In Vitro Adventitious Shoot Formation from Leaf Explants of Withania somnifera Dunal.

An efficient protocol was developed for high frequency plant regeneration from leaf explants of Withania somnifera Dunal on Murashige and Skoog (1962) medium supplemented with different concentrations of Auxins and cytokinins. Frequency of shoot bud regeneration varied with dose of plant growth regulators in the medium. Highest frequency of shoot buds was obtained at a concentration of 1.0 mg/l Kin. In vitro rooting of microshoots were obtained by growing them in half strength MS medium supplemented with 2.0 mg/l IBA. Rooted plantlets were successfully transferred to the field, after acclimatization in the net house.