Estimation of Vinblastine in Pharceutical Formulations by RP-HPLC Method

Vinblastine, a Vinka alkaloid and a chemical analogue of vincristine is generally used in combination therapy with synthetic molecules. A new simple, precise and accurate reverse phase HPLC method was developed and validated for the estimation of vinblastine in pharmaceutical formulations using the mobile phase 90% methanol, 5% acetonitrile and 5% of 0.1% orthophosphoric acid and the pH was maintained at 3.5. Analysis was carried out using PEAK 7000 isocratic HPLC with rheodyne manual sample injector with a switch (77251). The resultant chromatogram obtained has a high resolution and low tailing factor (1.29). The linearity curve showed a correlation coefficient (r2) of 0.999 for a wide range of drug concentrations of 3-15 ppm. The method was also validated in respect of precision, accuracy and specificity. The applicability of the method was also tested with commercial sample of Cytoblastin. This method is more accurate, precise and convenient in terms of linearity, accuracy, precision, and others.